Development of Ibuprofen-loaded Emulsion from Eutectic Mixture and Eudragit RL 100
The main objective of this study was to formulate an Ibuprofen loaded emulsion from the eutectic mixture and Eudragit RL 100. The eutectic mixture was formed with camphor and menthol which was approved by the FDA. A eutectic mixture is defined as a combination of two or more components at a certain ratio intermingle to form a new chemical compound resulting in a system having a lower melting point than either of the components. The formulated emulsion from the eutectic mixture was evaluated for various studies like pH checking, stability checking by centrifugation, viscosity, particle size analysis, quantitative drug analysis, thermodegradation and in-vitro drug release studies. Neutral pH values resulted in the formulations. The formulation was stable even after it was centrifuged for 5000 rpm for 30 min. Viscosity results showed the formulation was less viscous results in good spreadability. Particle size was determined with particle size analyzer. Drug analysis results revealed about 96 % of drugs are incorporated into the formulation. Thermodegradation results proved that there was no spillage of drugs from the formulations even when the drug was stored at different temperatures. In-vitro drug release study was performed by varying drug concentrations. The results showed there was no change in the drug release even after varying the drug concentration. From the results it was concluded that Ibuprofen was successfully formulated as an emulsion with the help of eutectic mixture a Eudragit RL 100.
2. British Pharmacopoeia (2000); Volume-I; British Pharmacopoeia Commission; London; 79-36-39.
3. British Pharmacopoeia (2013); British Pharmacopoeia Commission; London.
4. Cózar-Bernal M.J., Holgado M.A., Arias J.L., Muñoz-Rubio I., Martín-Banderas L., Alvarez-Fuentes J. and Fernández-Arévalo M (2011); Insulin-loaded PLGA microparticles: flow focusing versus double emulsion/solvent evaporation. J Microencapsul; 28(5); 430-441.
5. Dannie J.G.P. van Osch., Lawien F. Zubeir., Adriaan van den Bruinhorst., Marisa A.A. Rocha. and Maaike C. Kroon (2015); Hydrophobic Deep Eutectic Solvents as Water-Immiscible Extractants. Green Chem; 17; 4518-4521.
6. Indian Pharmacopoeia (2007); The Indian Pharmacopoeia Commission, Ghaziabad; Volume-I; 141-142. (IP, 2007)
7. Indian Pharmacopoeia (2010); The Indian Pharmacopoeia Commission, Ghaziabad; Volume-II; 1479.
8. Joseph Schwarz, and Michael Weisspapir (2005); Vehicle for topical delivery of anti-inflammatory compounds, available at https://patents.google.com/patent/CN100534530C/en
9. Marchlewicz A., Guzik U., Hupert-Kocurek K., Nowak A., Wilczyńska S. and Wojcieszyńska D (2017); Toxicity and biodegradation of ibuprofen by Bacillus thuringiensis B1(2015b); Environ Sci Pollut Res Int; 24(8); 7572-7584.
10. Mazaleuskaya L.L., Theken K.N., Gong L., Thorn C.F., FitzGerald G.A., Altman R.B. and Klein T.E (2015); PharmGKB summary: ibuprofen pathways; Pharmacogenet Genomics; 25(2); 96-106.
11. Patra C.N., Priya R., Swain S., Kumar Jena G., Panigrahi K.C. and Ghose D (2017); Pharmaceutical significance of Eudragit: A review; Future Journal of Pharmaceutical sciences; doi: 10.1016/j.fjps.2017.02.001.
12. Phaechamud T, and Tuntarawongsa S (2016); Transformation of eutectic emulsion to nanosuspension fabricating with solvent evaporation and ultrasonication technique. Int J Nanomedicine; 11; 2855-2865.
13. Ribeiro B. D., Florindo C., Iff L.C., Coelho M.A.Z. and Marrucho Ferreira I.M (2015); Menthol-based Eutectic Mixtures: Hydrophobic Low Viscosity Solvents. ACS Sustainable Chem. Eng. 3(10); 2469-2477.
14. Stanos S.P. (2007); Topical Agents for the Management of Musculoskeletal Pain; J Pain Symptom Manage; 33(3); 342-355.
15. Stanos S.P. and Galluzzi KE (2013); Topical Therapies in the Management of Chronic Pain; Postgrad Med; 2013; 125(4 sup1); 25-33.
16. Suresh C. Sikka. and Alma R. Bartolome (2018), “Chapter-36 Perfumery, Essential Oils, and Household Chemicals Affecting Reproductive and Sexual Health”. Suresh Sikka and Wayne Hellstrom, Bioenvironmental Issues Affecting Men's Reproductive and Sexual Health. Academic Press, 557-569.
17. Tamilvanan S. and Varinder K (2016); In Vitro Anti-inflammatory and Antimicrobial Activities of Azithromycin After Loaded in Chitosan-and Tween 20-Based Oil-in-Water Macroemulsion for Acne Management; AAPS PharmSciTech; 17(3); 700-709.
18. Tcholakova S., Denkov N., Ivanov I.B. and Marinov R (2004); Evaluation of Short-Term and Long-Term Stability of Emulsions by Centrifugation and NMR; Bulg. J. Phys; 31; 96-110.
19. The Japanese Pharmacopoeia (2001); Fourth Edition, 2001. Part-I; 75-77. (JP, 2001)
20. Weiser T., Richter E., Hegewisch A., Muse D.D. and Lange R (2018); Efficacy and safety of a fixed‐dose combination of ibuprofen and caffeine in the management of moderate to severe dental pain after third molar extraction; Eur J Pain; 22(1); 28-38.