Invitro studies on the effect of Terminalia Arjuna in Adipocyte 3T3 - L1 Cell Lines

  • Balasubramanian Sathyamurthy Ramaiah College of Arts, Science and Commerce
  • B Ranjitha REVA Institute of Science and Management
  • Dinesh Bhaskar REVA Institute of Science and Management
Keywords: Terminalia arjuna, Arjunolic acid, 3T3-L1cell line


The leaves of Terminalia Arjuna are commonly known as Arjuna. It is used as traditional medicinal plant distributed throughout the greater part of the Indian Peninsula along rivers and found in Sub-Himalayan tract, Chota Nagpur, Orissa, West Bengal, Punjab, Deccan and Konkan it has many medicinal properties like anti bacteria, anti inflammatory and used for the treatment of Asthama, Bronchities. These leaf extracts are found to have antioxidant, anticancer and antidiabetic activities. Out of many constituents arjunolic acid is the active principle which is responsible for antidiabetic effects. The present work was aimed to study the effect of Terminalia arjuna. Methanolic extracts on adipocyte 3T3-L1cell line and found the phytochemical compounds of Terminalia arjunaleaves have more inhibitory scavenging and more antidiabetic activities.

Author Biographies

Balasubramanian Sathyamurthy, Ramaiah College of Arts, Science and Commerce

Department of Biochemistry,
Ramaiah College of Arts, Science and Commerce,
Bangalore, Karnataka, India

B Ranjitha, REVA Institute of Science and Management

Department of Biochemistry,
REVA Institute of Science and Management,
Bangalore, Karnataka, India

Dinesh Bhaskar, REVA Institute of Science and Management

Department of Biochemistry,
REVA Institute of Science and Management,
Bangalore, Karnataka, India


Chang C.C, Lin K.Y, Peng K.Y, Day Y.J, Hung L.M (2016); Resveratrol exerts anti-obesity effects in high-fat diet obese mice and displays differential dosage effects on cytotoxicity, differentiation and lipolysis in 3T3-L1 cells. Endocr. J. 63(2); 169-178.

Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of Diabetes Mellitus. Diabetes Care 1997, 20(7); 1183-1197.

Ruiz-Ojeda FJ, Rupérez AI, Gomez-Llorente C, Gil A, Aguilera CM (2016); Cell Models and their Application for Studying Adipogenic Differentiation in Relation to Obesity. Int. J. Mol. Sci., 17(7); E1040.

Giri RR, Giri KR, Patel SS and Palandurkar KM (2012); Effect of Terminalia arjunain accelerating healing process of experimentally fractured tibia of rats; A preliminary study. Research Journal of Pharmaceutical, Biological and Chemical Sciences; 3(4); 417-425

Green, H, Kehinde O (1976); Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells; Cell; 7(1); 105–113.

Hemant R J, Bhutani K K (2002); Antioxidant properties of Indian Medicinal plants; Phytother. Res; 16(8); 771-773.

Kangas L; Bioluminescence of cellular ATP (1984); A new method for Evaluating cytotoxic agents in vitro. Med. Biol.; 62(6); 338–43.

Khaliq F, Parveen A, Singh S, Gondal R, Hussain ME, Fahim M (2013); Improvement in myocardial function by Terminalia Arjuna in streptozotocin- induced diabetic rats; possible mechanisms. J Cardiovasc Pharmacol Ther; 18(5); 481-489.

Kumar PJ, Clark M; Textbook of Clinical Medicine. Pub Saunders (London) 2002; 1099-1121.

Manna P, Sinha M, Pal P, Sil P C (2007); Arjunoic acid a tri terpenoid saponin ameliorates arsenic induced cytotoxicity in hepatocytes; Chemico boil interacts; 170(3); 187-200.

Manna P, Sinha M, Pal P, Sil P C (2008); Protection of arsenic induced testicular oxidative stress by arjunolic acid. Redox Rep; 13(2); 67-77.

Olatunde Farombi E, Olayide Alanni O, Godwin Emerole O (2002); Antioxidant and scavenging activities of flavanoid extract (Kolaviron) of Garcinia kola seeds; Pharma. Biol; 40(2); 107-116.

Padmaa M Paarakh, Terminalina Arjuna, Rox B W, Arn (2010); Terminalia arjuna (Roxb.) Wt. and Arn. : A Review; International journal of pharmacology; 6(5); 515-534.

Poulos S.P, Dodson M.V, Hausman G.J (2010); Cell line models for differentiation: Preadipocytes and adipocytes. Exp. Biol. Med; 235(10); 1185-1193.

Segelman A B, Fransworth N R, Quimbi M D, L Loide (1969); Biological and phytochemical evaluation of plants. Pub met; 32 (1); 52-58.

Sumitra M, Manikandan P, Kumar D.A, Arutselvan N, Balakrishna K, Manohar B.M, Puvanakrishnan R (2001); Experimental myocardial necrosis in rats; role of arjunolic acid on platelet aggregation, coagulation and antioxidant status; Mol. Cell Biochem; 224(1-2); 135-142.

Vishwanath D, Srinivasan H, Patil M.S, Seetarama S, Kumar S.A, Dixit M.N (2013); Novel method to differentiate 3T3 L1 cells in vitro to produce highly sensitive adipocytes for a GLUT4 mediated glucose uptake using fluorescent glucose analog; J. Cell Commun. Signal; 7(2); 129-140.

Wang H J, Jin Y X, Shen W, Neng J, Wu T, Li Y J, Fu Z W (2007); Low dose Streptozotocin (STZ) combined with high energy intake can effectively induce type-2 diabetes through altering the related gene expression; Asian Pac J. Clin Nutr.; 16(1); 412-417.

How to Cite
Sathyamurthy, B., Ranjitha, B. and Bhaskar, D. 2018. Invitro studies on the effect of Terminalia Arjuna in Adipocyte 3T3 - L1 Cell Lines. PharmaTutor. 6, 8 (Aug. 2018), 17-26. DOI:

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