Method development and validation of RP-HPLC method for the simultaneous estimation of Paracetamol and Eperisone Hydrochloride in Pharmaceutical Dosage Forms
Abstract
A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the simultaneous estimation of Paracetamol and Eperisone Hydrochloride in pharmaceutical Tablet dosage form. The mobile phase consisted of 60:40 % (v/v) of Methanol and 0.1% v/v orthophosphoric acid operated on isocratic mode. The flow rate is 1.0 ml/min. Chromatographic separation of Paracetamol and Eperisone Hydrochloride was performed on PHENOMENEX C18 column (150 X 4.6 cm, ODS, 5μm). The wavelength of detection is 270 nm. The injection volume is 20μL. The retention time of Paracetamol and Eperisone Hydrochloride are 2.21 ± 0.10 minutes and 2.74 ± 0.10 respectively. The run time of analysis is 6 minutes. The developed method was validated for parameters such as accuracy, precision, linearity, limit of detection, limit of quantitation. The influence of acid, alkaline, oxidative Stress and photolytic stress conditions on both the drugs was studied. Results indicated partial degradation in alkaline medium for Paracetamol and Eperisone Hydrochloride. The proposed method has been successfully used for the estimation in tablet dosage forms
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