Development and Validation of HPTLC Method for Estimation of Tapentadol Hydrochloride

Shoumik Roy; Samil Desai; Bhavna Patel; Shraddha Parmar

Shoumik Roy Sardar Patel University
Samil Desai Sardar Patel University
Bhavna Patel Sardar Patel University
Shraddha Parmar Sardar Patel University

Keywords: Tapentadol Hydrochloride, HPTLC Method, Analgesic Effect

Abstract

The main thrust of the paper was to develop and validate a simple, precise high performance thin-layer chromatographic (HPTLC) method for estimation of tapentadol hydrochloride in tablet dosage form. Chromatography was performed on silica gel 60 F₂₅₄ plates with Chloroform: Acetone: Ammonia (2.5: 2.4: 0.1 v/v/v) as mobile phase. This mobile phase system gave a good resolution for tapentadol hydrochloride (R_f value of 0.49 ± 0.02). Detection and quantification were carried out at 272 nm. The linear regression data for the calibration plot showed a good relationship with r=0.999. The limits of detection and quantification were 62.68 and 189.94ng/spot for tapentadol hydrochloride. The amounts of the drugs in the marketed formulation were 99.98%.

Author Biographies

Shoumik Roy, Sardar Patel University

Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University,
Vallabh Vidyanagar, Gujarat, India.

Samil Desai, Sardar Patel University

Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University,
Vallabh Vidyanagar, Gujarat, India.

Bhavna Patel, Sardar Patel University

Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University,
Vallabh Vidyanagar, Gujarat, India.

Shraddha Parmar, Sardar Patel University

Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University,
Vallabh Vidyanagar, Gujarat, India.

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