Development of High Performance Thin Layer Chromatography Method for the Determination of Scopolin in Convolvulus Pluricaulis Chois

Babu Lal

Babu Lal Indo-Soviet Friendship College of Pharmacy

Keywords: Shankhpushpi, Standardization, Validation, Scopolin

Abstract

A simple, selective, precise and robust high performance thin layer chromatography (HPTLC) method was developed and validated for the determination of scopolin, in Convolvulus pluricaulis, commonly known as shankhpushpi. Aluminium backed pre-coated TLC plates (Silica gel 60F-254) of 0.2 mm thickness, were utilized to perform chromatography. The plates were developed up to 90 mm distance in twin trough glass chamber, saturated for 10 min with mobile phase system (Chloroform: methanol, 8.5:1.5, v/v). The system produces a sharp and well-defined peak for scopolin (R_f value; 0.30 ± 0.02). Scopolin was quantified in various test samples (extracts and marketed formulation) at absorbance maxima of 340 nm with the help of CAMAG TLC scanner III. The linear regression analysis data for the calibration plots (concentration Vs peak area) showed good linearity (r² = 0.9992 ± 0.0002) in the concentration range of 20-160 ng spot⁻¹. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines. The statistical analysis of the results, indicate that the proposed method is specific, accurate and precise. Further, this method can be used for the standardization of shankhpushpi and its formulations.

Author Biography

Babu Lal, Indo-Soviet Friendship College of Pharmacy

Department of Pharmacognosy,
Indo-Soviet Friendship College of Pharmacy,
Moga, Punjab, India

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