RP-HPLC Method for the Estimation of Nitaoxanide in Pharmaceutical Formulation
Keywords:
Verticillium Teneru, Acremonium, VLC, HPLC
Abstract
Objective: A simple and precise RP-HPLC method was developed and validated for the determination of Nitaoxanide in pharmaceutical dosage forms.
Materials and Methods: Chromatography was carried out using waters RP –C18 150×4.6 mm, 3.5 µ, pH 6.8, buffer: acetonitrile (50:50) as the mobile phase at a flow rate 1.2 ml/min. The analyze was monitored using PDA detector at 254 nm. The proposed method was found to have linearity in the concentration range of 25-150µg/ml with correlation co efficient of r2 =0.9999.
Results: The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for Nitaoxanide were in the range 100.06-101.9%.
Conclusion: Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining Nitaoxanide in bulk and dosage forms.
References
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3. Singh H.P.,Sharma C.S.,Amar Deep Angali., Agalann S.K.,Ranawat M.S., Spectrophotometric methods for simultaneous determination of Nitazoxanide and Ofloxacin in combined bulk and pharmaceutical formulations; International Journal of Pharm Tech Research; 2011;3:118-123.
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8. Sharma MS., Sharma MC.; Development of visible spectrophotometric methods for the estimation of nitazoxanide in bulk and pharmaceutical formulation using ferric chloride; American-Eurasian Journal of Scientific Research; 2011;6(3):155-160.
9. VidyaSagar., Syed AzharNizami., Gurumurthy., Ramesh M.R; Colorimetric Estimation of Nitazoxanide and Ofloxacin in Pharmaceutical Dosage from; International Journal of Pharma Research and Development; 3:199-206.
10. LopamudraAdhikar., SanjivSahu., SarbeswarJagdev; Development and validation of spectro photo metric and colorimetric method for the determination of Nitazoxanide in its bulk and pharmaceutical dosage form (Tablet);International Journal of Chem Tech Research; 2011;3(1):131-135.
11. Marcelo Donald Malesuik., ClesioSoldatelliPaim., Elfrides Eva Scherman, Schapovale, Martin Steppe; Development of a simple, rapid and validated spectrophotometric method for Nitaoxanide in pharmaceutical formulation and comparison with HPLC; Quim.nova;2010;33:3
12. Sharma M.C., Sharma M.C; Development and validation of a dissolution method with isocratic high performance liquid chromatographic determination of Nitaoxanide and ofloxacin in pharmaceutical dosage form; American Eurasian Journal of Scientific Research;2011, 6(3):155-160.
13. Hanna S. El-Desoky., Mohamed M. Ghoneim and Mohamed M. Abdel-Galeil., Stripping Volumetric methods for determination of the Ant parasitic drug Nitaoxanide in bulk form, Pharmaceutical formulation and human serum; J . Braz.Chem.Soc; 2010, 21(4):669-679.
14. Gopu C.L., Shibu Thomas., Paradkar A.R., Mahadik K.R; A validated stability indicating HPTLC method for the determination of nitazoxanide; Journal ofscientific and industrial Research; 2007, 66:141-145.
15. Siva Kumar R., Kumar Nallasivan P., Saravanakumar S., Kandasamy and Venkatnarayanan R; Simultaneous RP-HPLC estimation of Nitazoxanide and Ofloxacin in tablet dosage forms; Asian Journal of Research Chem; 2009; 2(1):43-45.
16. Premanand D.C., Senthilkumar K.L., Senthilkumar B., Saravanakumar M., Thirumurthy R; A validated RP-HPLC method for simultaneous estimation of nitazoxanide and ofloxacin in pharmaceutical formulations; Pelagia Research Library; 2010;1(2):1-5.
17. Sharna S., Bhandari A., Choudhari V.R., Rajpurohit H; RP-HPLC method for simultaneous estimation of nitazoxanide and ofloxacin in tablets; Indian Journal of Pharmaceutical Sciences; 2011, 84-88.
18. Katla R., Sharma R., Chaturvedi S; Simultaneous RP HPLC determination of nitazoxanide and ofloxacin in combined tablet dosage form(Short communication) (Reverse phase high performance liquid chromatography)(clinical report); Indian Journal of Pharmaceutical Sciences; 2008; 1:38.
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2. Prabhakar G, Kapse, Appala G.K., Spectrophotometric determination of Nitazoxanide in pharmaceutical dosage form. Indian Journal of Pharmaceutical Sciences; 2006, 3(68):403 – 406.
3. Singh H.P.,Sharma C.S.,Amar Deep Angali., Agalann S.K.,Ranawat M.S., Spectrophotometric methods for simultaneous determination of Nitazoxanide and Ofloxacin in combined bulk and pharmaceutical formulations; International Journal of Pharm Tech Research; 2011;3:118-123.
4. Vanitha J., Kumar A., Saravana., Ganesh M., Vetrichelvan T., Estimation of Nitazoxanide by UV Spectrophotometric method; Journal of Global Pharma Technology;2009, (1): 51-53.
5. Senthilraja., Simultaneous UV Spectrophotometric method for the estimation of nitazoxanide and ofloxacin in combined dosage form; Research J.Pharm.and Tech; 2008;1(4):469-471.
6. Shailendra Bindaiya., Shravan Bankey., Deepi Jain., Simultaneous determination of nitazoxanide and ofloxacin in tablet by ultraviolet spectrophotometry (dual wavelength method); International Journal of Chem Tech Research; 2;2010:11-15.
7. Lakshminarayana KV., Manohara YN., Gurupadayya BM; Indian Journal of Pharmaceutical Sciences; 2007, 69(1):147-149.
8. Sharma MS., Sharma MC.; Development of visible spectrophotometric methods for the estimation of nitazoxanide in bulk and pharmaceutical formulation using ferric chloride; American-Eurasian Journal of Scientific Research; 2011;6(3):155-160.
9. VidyaSagar., Syed AzharNizami., Gurumurthy., Ramesh M.R; Colorimetric Estimation of Nitazoxanide and Ofloxacin in Pharmaceutical Dosage from; International Journal of Pharma Research and Development; 3:199-206.
10. LopamudraAdhikar., SanjivSahu., SarbeswarJagdev; Development and validation of spectro photo metric and colorimetric method for the determination of Nitazoxanide in its bulk and pharmaceutical dosage form (Tablet);International Journal of Chem Tech Research; 2011;3(1):131-135.
11. Marcelo Donald Malesuik., ClesioSoldatelliPaim., Elfrides Eva Scherman, Schapovale, Martin Steppe; Development of a simple, rapid and validated spectrophotometric method for Nitaoxanide in pharmaceutical formulation and comparison with HPLC; Quim.nova;2010;33:3
12. Sharma M.C., Sharma M.C; Development and validation of a dissolution method with isocratic high performance liquid chromatographic determination of Nitaoxanide and ofloxacin in pharmaceutical dosage form; American Eurasian Journal of Scientific Research;2011, 6(3):155-160.
13. Hanna S. El-Desoky., Mohamed M. Ghoneim and Mohamed M. Abdel-Galeil., Stripping Volumetric methods for determination of the Ant parasitic drug Nitaoxanide in bulk form, Pharmaceutical formulation and human serum; J . Braz.Chem.Soc; 2010, 21(4):669-679.
14. Gopu C.L., Shibu Thomas., Paradkar A.R., Mahadik K.R; A validated stability indicating HPTLC method for the determination of nitazoxanide; Journal ofscientific and industrial Research; 2007, 66:141-145.
15. Siva Kumar R., Kumar Nallasivan P., Saravanakumar S., Kandasamy and Venkatnarayanan R; Simultaneous RP-HPLC estimation of Nitazoxanide and Ofloxacin in tablet dosage forms; Asian Journal of Research Chem; 2009; 2(1):43-45.
16. Premanand D.C., Senthilkumar K.L., Senthilkumar B., Saravanakumar M., Thirumurthy R; A validated RP-HPLC method for simultaneous estimation of nitazoxanide and ofloxacin in pharmaceutical formulations; Pelagia Research Library; 2010;1(2):1-5.
17. Sharna S., Bhandari A., Choudhari V.R., Rajpurohit H; RP-HPLC method for simultaneous estimation of nitazoxanide and ofloxacin in tablets; Indian Journal of Pharmaceutical Sciences; 2011, 84-88.
18. Katla R., Sharma R., Chaturvedi S; Simultaneous RP HPLC determination of nitazoxanide and ofloxacin in combined tablet dosage form(Short communication) (Reverse phase high performance liquid chromatography)(clinical report); Indian Journal of Pharmaceutical Sciences; 2008; 1:38.
19. ICH-Q2B, Validation of analytical procedure. Methodology, ICH. Harmonized Tripartite Guideline, Geneva, 1996, 1-8.
20. ICH-Q2A, Text on validation of analytical procedures, ICH. HarmonizedTripartite Guideline, Geneva, 1995, 2-3, A-1 to A-3.
Published
2014-12-01
How to Cite
[1]
Meera, R., Swathylakshmi, N., Sundarapandian, M., Pandian, R. and allayasamy, M. 2014. RP-HPLC Method for the Estimation of Nitaoxanide in Pharmaceutical Formulation. PharmaTutor. 2, 12 (Dec. 2014), 145-149.
Section
Articles
