In vitro Membrane Stabilizing and Insecticidal Activities of Methanolic Extract of Streblus asper Lour

Abstract

We aimed at assessing the effect of methanolic extract of Streblus asper in human red blood cell (HRBC) membrane stabilization and insecticidal (on the stored grain pest, Trogoderma  granarium Everts) as studies. The membrane stabilizing activity was assessed by using erythrocyte in hypotonic solution and heat induced was compared with acetyl salicylic acid. The extract at the doses of  200, 400 and 800 μg/ml significantly inhibited heat induced lysis of the human red blood cell membrane with values of 46.53%, 56.52% and 65.14%, respectively. The results of hypotonic solution induced lysis showed that S. asper has significant reduction (P≤0.01) in inflammation i.e. 40.8 % (400 µg/ml) and 50.8 % (800 µg/ml) as compared to the standard drug, acetyl salicylic acid, which was 62.96 % in insecticidal assay the extract showed dose dependent paralyzing effect and mortality of T. granarium Everts. All the doses of crude extracts exhibited concentration and time dependent insecticidal activity.