Development and Validation of Analytical Method for Simultaneous Estimation of Lamotrigine and Clozapine in Synthetic Mixture by Absorbance Correction Method
Keywords:
Abrasive action, fluoride, human health, kidney disease, reproductive functioning
Abstract
The simple spectroscopic method has been developed for simultaneous estimation of Lamotrigine and Clozapine in synthetic mixture. Absorbance Correction Method involves the measurement of absorption at two wavelengths 307 nm (lmax for Lamotrigine) and 360 nm (lmax for Clozapine). The method was found linear between the range of 1-5 µg/ml for Lamotrigine and 6-30 µg/ml for Clozapine for method .The accuracy and precision was determined and validated statistically. Both the method showed good reproducibility and recovery with %RSD less than 1.The method was found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis for Lamotrigine and Clozapine in bulk and combined dosage form.
References
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2. Cohen AF, Land GS, Breimer DD, Yuen BC, Winton PC and Peak AW.; Lamotrigine, a new anticonvulsant: Pharmacokinetics in normal humans; Clini Pharmacol Thera; 1987; 42(5); 535-541.
3. Manoj G Tyagi; Estimation of Clozapine in Human Plasma by High – Performance Liquid Chromatography and detection by UV-VIS detector; Asian J of Biochem and Pharm Res; 2012; 2(2); 49-53.
4. IUPAC Name available from : Clozapine _ C18H19ClN4 _ ChemSpider.htm
5. Manjunatha JG, Kumara Swami BE, Mamatha GP, Umesh C, Niranjana E & Sherigara BS; Cyclic Voltammetric Studies of Dopamine at Lamotrigine and TX-100 Modified Carbon Paste Electrode; Int J Electro Sci; 2009; 4(2); 187-196.
6. James WJ. Lamotrigine in Psychiatry Pharmacology and Therapeutics.CNS Spectr, 2005; 10(3); 224-232.
7. Large CH, Webster EL & Goff DC; The potential role of Lamotrigine in schizophrenia; Psychopharmacology; 2005; 181(3); 415-436.
8. Maurice D, Chao C.; Lamotrigine: Chemistry, Bioinformation and Pharmacokinetics; Antiepileptic drugs; 2002; 5; 370-385.
9. Somayeh J, Francesca F, Xu-Feng H; Structural Contribution of antipsychotic drugs to their therapeutic profiles and metabolic side effect; J Neurochem; 2012; 120(3); 371-384.
10. International Conference on Harmonization, Harmonized Tripartite Guideline, Validation of Analytical Procedures Text and Methodology, ICH Q2 (R1), 2005
Published
2015-06-01
How to Cite
[1]
Atodariya, P., Raj, H. and Jain, V. 2015. Development and Validation of Analytical Method for Simultaneous Estimation of Lamotrigine and Clozapine in Synthetic Mixture by Absorbance Correction Method. PharmaTutor. 3, 6 (Jun. 2015), 41-48.
Section
Articles
