Difference spectrophotometric estimation of Capecitabine from tablet dosage form
Keywords:
Capecitabine, Spectroscopy, Spectrophotometer
Abstract
Simple, sensitive and specific spectrophotometric method were developed and validated for quantification of CAPECITABINE (CAP) by difference spectroscopy. CAP exhibits a substantial difference in absorbance in the two solvents that is in 0.01 N HCL and 0.01 N NAOH at 295 nm. Beer’s law was obeyed in the concentration range of 1 to 20 μg ml-1for CAP. Results of tablet analysis showed standard deviation in the range of 98.42 to 101.95 % for CAP which indicate repeatability of the method. The results indicated excellent recoveries ranging from 98.45 to 101.70 % for CAP with a mean of 99.12 %. Recoveries obtained do not differ significantly from 100% showed that there was no interference from the common excipients used in the tablet formulation indicating accuracy and reliability of the method.
References
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2. Herman J.M.; Analysis of Fluorouracil-Based Adjuvant Chemotherapy and Radiation after pancreatic oduodenectomy for Ductal Adenocarcinoma of the pancreas; Journal of Clinical Oncology; 2008; 26(21); 3503-3510
3. VanCutsem E.; A phase IB dose-escalation study of Erlotinib, Capecitabine and Oxaliplatin in Metastatic Colorectal Cancer Patients; Ann Oncology; 2008; 19(2); 332-339
4. Neoptolemos J.P.; A Multicenter, International, Open-Label, Randomized, Controlled Phase III trial of adjuvant 5-fluorouracil/folinic acid (5-fu/fa) versus Gemcitabine (gem) in patients with Restricted Pancreatic Ductal Adenocarcinoma; Journal of Clinical Oncology; 2009; 27(18s); LBA4505
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6. Twelves C.; Erlotinib in combination with Capecitabine and Docetaxel in patients with Metastatic Breast Cancer: a dose- escalation study; European Journal of Cancer; 2008; 44(3); 419-426
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9. Zufia L.; Simple Determination of Capecitabine and its metabolites by Liquid Chromatography with Ultraviolet Detection in a single injection; Journal of chromatography B; 2004; 809(1); 51- 58
10. Sylvie M.; Simultaneous Determination of Capecitabine and its metabolites by HPLC and Mass Spectrometry for Preclinical and Clinical studies; Journal of chromatography B; 2005; 826(1-2); 232-237
11. Wang S; Development of a sensitive and selective LC/MS/MS method for the determination of α-fluoro-β-alanine-5-fluoro uracil and Capecitabine in human plasma; Journal of chromatography B; 2005; 265; 32-37
12. Xu Yan; Liquid Chromatography-Mass Spectrometry method for the analysis of the anti-cancer agent Capecitabine and its nucleoside metabolites in human plasma; Journal of chromatography B; 2003; 783(1), 273- 285
13. Desmoulin F.; Isolaton of an unknown metabolite of Capecitabine, an oral 5-fluorouracil prodrug, and its identification by Nuclear Magnetic Resonance and Liquid Chromatography-Tandem Mass Spectrometry as a glucuroconjugate of 5’- deoxy-5-fluorocytidine, namely 2’-(β-Dglucuronic acid)-5’-deoxy-5-fluorocytidine; Journal of chromatography B; 2003; 792(2); 323-332
14. Gutens G.; Hyphenated Techniques in Anticancer Drug Monitoring II. Liquid Chromatography-Mass Spectrometry and Capillary Electrophoresis; Journal of chromatography A; 2002; 976(1-2); 239-247
15. Patil P.S., More H.N.; Difference spectroscopic estimation of Enalapril maleate from tablet dosage form; International journal of Research in Pharmaceutical and Biomedical Sciences; 2011; 2(2); 629-633
16. Sankpal Poournima., Doijad Rajendra, Suryawanshi Gayatri, Patil Priyanka, Pathan Aasma; Simple Quantitative Method Development and Validation of Capecitabine in Pure Form and Pharmaceutical Dosage Forms by UV-Spectroscopy; 2012 (1).
17. ICH guideline Q2 (R1); Validation of Analytical Procedures: Text and Methodology; 2005
Published
2016-03-01
How to Cite
[1]
Doijad, R., Patil, R., Lade, P. and Sankpal, P. 2016. Difference spectrophotometric estimation of Capecitabine from tablet dosage form. PharmaTutor. 4, 3 (Mar. 2016), 17-21.
Section
Articles
