Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique

Abstract

Isolation of Nucleic acid easily by Solid Phase Extraction (SPE) and this approach, using commercially available Extraction of nucleic acid column-based kits, requires no toxic chemicals and is a rapid and consistent method for concomitant protein extraction. It is a modern technique useful for separation of Nucleic acid which is most reliable, less time consuming and separation of impurities and continuity of reactive products using listed techniques like column-based nucleic acid purification, Nucleic acid methods and ethanol precipitation, DNA separation by silica adsorption. Buffer choice is significant to completely solubilized all proteins in the sample. This technique provides a simple and effective way to analyze protein and nucleic acids simultaneously from the same sample not affecting yield and quality.