Estimation of Lacidipine in Bulk and Pharmaceutical Dosage Form by Zero Order, and First Order Derivative UV Spectrophotometric Methods

  • Girija Dandu ASSISTANT PROFESSOR
  • Padma Akkimi Sankar Reddy Institute of Pharmaceutical sciences
  • A.Tirupathi Reddy Sankar Reddy Institute of Pharmaceutical sciences
  • Basu Venkateswara Reddy Sankar Reddy Institute of Pharmaceutical sciences
Keywords: Lacidipine, Zero order, first order, UV Spectroscopy, methanol, Validation

Abstract

The present research work was broadly focused on the estimation of in Lacidipine in bulk and pharmaceutical dosage form by using two UV spectrophotometric methods like Zero order spectroscopy (Method-1), and first order derivative spectroscopy (Method-2). The solvent employed for the two methods was methanol and absorption maximum was found to be 284nm, and 277nm respectively. The developed methods showed linearity sin the range between 20-100µg/ml. The correlation co-efficient was ≥ 0.999. The precision (%RSD) for all the two methods was found to be ≤2. The accuracy was performed by spiking standard drug at 50,100 and 150% of the test concentration and the values obtained were within the limits. The assay for the formulation was found to be within limits. All the results were satisfactory the developed methods were linear, accurate, reproducible and robust.

Author Biographies

Padma Akkimi, Sankar Reddy Institute of Pharmaceutical sciences

Department of Pharmaceutical Analysis,
Sankar Reddy Institute of Pharmaceutical sciences,
Salakalveedu, Prakasam, Andha Pradesh, India

A.Tirupathi Reddy, Sankar Reddy Institute of Pharmaceutical sciences

Department of Pharmaceutics,
Sankar Reddy Institute of Pharmaceutical sciences,
Salakalveedu, Prakasam, Andha Pradesh, India

Basu Venkateswara Reddy, Sankar Reddy Institute of Pharmaceutical sciences

Department of Pharmaceutics,
Sankar Reddy Institute of Pharmaceutical sciences,
Salakalveedu, Prakasam, Andha Pradesh, India

References

Beckett AH, Stenlake JB. (1997); Practical Pharmaceutical Chemistry; 4th Ed. CBS Publishers and Distributors ; New Delhi

Chatwal, Anand (2000); Instrumental Methods of Chemicals Analysis ; 1st ed. Himalaya Publishing House; Mumbai

David Harvey (1997); Modern Analytical Chemistry 1st ed; Harcourt Brace & Company; United States of America

Day RA, Underwood AL (1986); Quantitative Analysis; 4th Ed. Prentice Hall New Delhi

Elhance DN. (2003); Fundamentals of statistics; Kitabmahal

Foster A, Hempenstall J, Tucker I, Rades T (2001); Selection of excipients for melt extrusion with two poorly water-soluble drugs by solubility parameter calculation and thermal analysis. Int J Pharm; 226(1-2); 147-161.

http://en.wikipedia.org/wiki/Lacidipine.

http://www Elchem.kaist.ac.kr/vt/chem.-ed/sep/lc/hplc.htm.

http://www.drugs.com/international/lacidipine.html

Nozal Maria J, Bernal Jose L, Jimenez Juan J, Martin Maria T, Diez Francisco J (2004); Development and validation of a liquid chromatographic method for determination of Lacidipine residues in surfaces in the manufacturer of Pharmaceuticals. J Chromatogr ; 1024(1); 115-122.

Ravi Sankar S. (2001); Text book of pharmaceutical analysis; 3rd ed. Rx Publications; Tirunelveli

Scott RPW, Simpson CF, Kadz ED. (1924); Tandem techniques; John Wileyand Sons, Inc; New York

Sharma BK (2000); Instrumental Methods of Chemical Analysis 13th ed; Goel Publisher House; Meerut

Willard HH, Merrit LL, Dean JA, Settle FA. Jr. (1989); Instrumental Methods of Analysis. 6th Ed. CBS Publishers and Distributors ; New Delhi

Yuri Kazakevich, Rosario Lobrutto. (2007); HPLC for pharmaceutical scientists; A John Wiley and Sons, Inc ; Publication United States of America

Published
2019-03-01
How to Cite
[1]
Dandu, G., Akkimi, P., Reddy, A. and Reddy, B.V. 2019. Estimation of Lacidipine in Bulk and Pharmaceutical Dosage Form by Zero Order, and First Order Derivative UV Spectrophotometric Methods. PharmaTutor. 7, 3 (Mar. 2019), 73-78. DOI:https://doi.org/10.29161/PT.v7.i3.2019.73.
Section
Articles