Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method
Keywords:
Diclofenac sodium, UV visible spectrophotometry, AUC, Method Validation
Abstract
A simple, precise, accurate and economical UV visible spectrophotometric method has been developed for estimation of Diclofenac sodium drug by AUC method. The standard and sample solutions were prepared by using double distilled water as a solvent. Quantitative determination of the drug was performed at wavelength range 270-282 nm. The linearity was established over the concentration range of 05, 10, 15, 20 & 25 μg/ml for Diclofenac sodium with correlation coefficient value of 0.9981. Precision studies showed that % relative standard deviation was within range of acceptable limits. The mean percentage recovery was found to be 99.38%.The proposed method has been validated as per ICH guidelines.
References
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2. Rajesh Sharma, Geetam Pathodiya And Ganesh Prasad Mishra; A Novel Application of Hydrotropic Solubilization In Development and Validation of Spectrophotometric Method For Simultaneous Estimation of Paracetamol and Diclofenac Sodium In Solid Dosage Form; International Journal of Pharma and Bio Sciences; Jul- Sep; 2010; 1(3); 1-9
3. Aravapalli Vasavi, D.Visagaperumal; Development and Validation of RP-HPLC method for simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in Pharmaceutical Dosage Form; Adv. Pharm. Edu. & Res; 2013; 3(4); 550-555.
4. International Conference on Harmonization (ICH) of Technical Requirements for the registration of Pharmaceuticals for Human use, Validation of Analytical Procedures Methodology; ICH-Q2(R1); Geneva; 1996; 1- 8
Published
2015-04-01
How to Cite
[1]
Digambar, M., Santosh, J., Pandurang, M. and Ashpak, T. 2015. Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method. PharmaTutor. 3, 4 (Apr. 2015), 21-25.
Section
Articles
